Coding
Part:BBa_K209486:Design
Designed by: Katja Kolar Group: iGEM09_UCSF (2009-10-01)
AarI B-D part, DOR-Actinin1ABD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 31
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 31
Illegal NotI site found at 1010 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 641
Illegal BglII site found at 1286 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 31
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 31
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Cloned into pCR2.1.
Source
Parent plasmid obtained from von Zastrow lab, UCSF.